Insertion junction: CLIP2.055393_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.055393
Chromosome:chromosome 2
Location:6952284
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g389208 (1 of 2) PTHR11266//PTHR11266:SF17 - PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17 // PROTEIN MPV17 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGACTGGTGCAGCGCGCTGGCCTGGTCCTCAGGCCATCATGCATATCG
Internal bar code:GCTCAACCCCTAAATTATTGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:3437
LEAP-Seq percent confirming:20.6897
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:23
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:TGGCGCCAGCTAGAATCAAA
Suggested primer 2:CTGCCTAGCCAGACATACCG