| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.055411 |
| Chromosome: | chromosome 16 |
| Location: | 3606363 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g672041 | (1 of 1) 2.1.1.228 - tRNA (guanine(37)-N(1))-methyltransferase / tRNA-(N(1)G37) methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAAACCCCCTCTCTCGGCACGCGCACAGGTTTACGCTGGACCGGCCTC |
| Internal bar code: | CGGAGAACATCGCCTCACGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3676 |
| LEAP-Seq percent confirming: | 97.0149 |
| LEAP-Seq n confirming: | 65 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 67 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACTTACCTGGTGCGTCTG |
| Suggested primer 2: | CGACTGTTCAGACACCGACA |