Insertion junction: CLIP2.055428_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.055428
Chromosome:chromosome 17
Location:328258
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g698200 MMS21 SUMO ligase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCAGGCTGCAGCCACTTCCTGCGCGAGGAGCAGCTGGTGCCGGCGGCGG
Internal bar code:CAGTACCGTGGTAAGCTTTGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:156
LEAP-Seq percent confirming:6.66667
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:ATCACCTAGCCAACCCTTGC
Suggested primer 2:TGCATGCATGTGTGTGTGTG