| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.055435 |
| Chromosome: | chromosome 7 |
| Location: | 3561391 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g336600 | AMD1,MAN1 | (1 of 1) K01230 - mannosyl-oligosaccharide alpha-1,2-mannosidase (MAN1); Alpha-1%252C2-mannosidase I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGTCAACGGATTGGGTTGGTTGCCGGGACTCAGCCGCCTCCCCCGCGT |
| Internal bar code: | TTTTGTGGGCCGTCCCTATTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 468 |
| LEAP-Seq percent confirming: | 41.4634 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCACAGCAGTTGAAAGCC |
| Suggested primer 2: | TGGACTTGATGATGGGCCAC |