| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.055437 |
| Chromosome: | chromosome 3 |
| Location: | 5310850 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g182700 | (1 of 1) PTHR31832//PTHR31832:SF3 - FAMILY NOT NAMED // B-BOX ZINC FINGER PROTEIN 20 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCCGCAGTTGGCGCAGCGGCACGTGCGCGTCCCTTGCCAGTCCTGCCC |
| Internal bar code: | CTCAGAATGACAGCGTCCGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4474 |
| LEAP-Seq percent confirming: | 93.75 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAATGTGTCCAGCAGCATG |
| Suggested primer 2: | CACAGCGTCAATAAGGTGCG |