| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.055440 |
| Chromosome: | chromosome 12 |
| Location: | 4774052 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g523250 | MAE15 | (1 of 11) K03327 - multidrug resistance protein, MATE family (TC.MATE, SLC47A, norM, mdtK, dinF); MATE efflux family protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGAGCCCAGGTAGCCCAGGCGCAGCACGTACACCAGCACGTAGTTGTT |
| Internal bar code: | TCAGTCCCGTTGCTTGTCTAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2236 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAGAGTAGACGCGTTGTT |
| Suggested primer 2: | ACAGGATGCCGTACAGGTTG |