Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | - |
Strain: | CLIP2.055459 |
Chromosome: | chromosome 2 |
Location: | 6013077 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre02.g118650 | PHC47 | Pherophorin-chlamydomonas homolog 47; (1 of 71) PF12499 - Pherophorin (DUF3707) | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGTTCAAGGGCGCCACGGCGGCCGCCGGCAGCAGCAGCGGCGGCGCCA |
Internal bar code: | TTGCTGCACACATTCGAAACCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1265 |
LEAP-Seq percent confirming: | 85.7143 |
LEAP-Seq n confirming: | 18 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CACCTCTACCACAAGGCTCG |
Suggested primer 2: | GTCACACCTCGTCTTCACGT |