| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.055470 |
| Chromosome: | chromosome 11 |
| Location: | 683674 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467620 | CEP83 | Centrosomal protein 83 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTTGTCCCTGGCTGTTCCAACGGCTGAATGGCTTGGCGTGCCTGATAT |
| Internal bar code: | CATCTTCGTAATACAGTATAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 818 |
| LEAP-Seq percent confirming: | 78.5714 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAATCAGGGGCGTGAAGAGG |
| Suggested primer 2: | TTGACGTGTCAAGATGGGGG |