Insertion junction: CLIP2.055497_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.055497
Chromosome:chromosome 12
Location:2267195
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g508500 (1 of 2) 3.4.17.4 - Gly-Xaa carboxypeptidase / Glycine carboxypeptidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTTCATACGACCGCTGTACTGTACAATGACTTGGGGTTTGTCTTACGTC
Internal bar code:CTTTAGGGTTATCGGTATTGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:1830
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:TGTGTTGTGTGATGGCCTGT
Suggested primer 2:ATTAATTGCCGACGGGGGTT