| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.055518 |
| Chromosome: | chromosome 12 |
| Location: | 1547242 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g487600 | CPS2 | Putative cleavage and polyadenylation specificity factor 100 kDa subunit; (1 of 1) K14402 - cleavage and polyadenylation specificity factor subunit 2 (CPSF2, CFT2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCCAAGGGAAGGGGCGTGTCTTTGGAACTCTGCCCAAAGAGTCCCTGT |
| Internal bar code: | TGTATGCTTTCAACTCTGTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 86 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCCTGTACCTCTCCCCCA |
| Suggested primer 2: | GCGCCACACTAAACGTTTGT |