Insertion junction: CLIP2.055525_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.055525
Chromosome:chromosome 2
Location:5348084
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g113400 PACRG1,PACRG,BUG21 Parkin Co-Regulated Gene Protein; (1 of 1) PTHR21207:SF2 - PARKIN COREGULATED GENE PROTEIN intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTATAGAAAGCTCTGCTCATTCAAGAGCCTTGTGACTTCGATGATGCGCG
Internal bar code:TTCATCTACACAACGCTTCTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2913
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:30
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:GCTATGCGCTTGTACTGTGC
Suggested primer 2:GATGATGAGCTGCGGGATGA