| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.055534 |
| Chromosome: | chromosome 13 |
| Location: | 2276143 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g578550 | (1 of 3) IPR000104//IPR011009 - Antifreeze protein, type I // Protein kinase-like domain | intron | |
| lncRNA_TCONS_00020366 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGCACTTCCCCCTGTGGTAACCCATGTATCCGTTGCTCCCTGAAGATG |
| Internal bar code: | AATGGGCACCTTAACGATGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1876 |
| LEAP-Seq percent confirming: | 27.1429 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 51 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATCTGAGCTGACTGGGCCT |
| Suggested primer 2: | ATCTGGGCGCAGATGATCAG |