| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.055535 |
| Chromosome: | chromosome 13 |
| Location: | 4286692 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g592250 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAAGCAAATGAGCCCGAGCGCTTGCAGTCTACGGCGAGGCGGTGGCCC |
| Internal bar code: | TTTGCACCAACACAGCAGTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3748 |
| LEAP-Seq percent confirming: | 84.7826 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGCGCAATACTGAAGCCA |
| Suggested primer 2: | TCAAATGAAGAGTGCGCCCT |