Insertion junction: CLIP2.055536_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.055536
Chromosome:chromosome 2
Location:3771525
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g100850 (1 of 1) IPR000104//IPR005828//IPR011701//IPR020846 - Antifreeze protein, type I // Major facilitator, sugar transporter-like // Major facilitator superfamily // Major facilitator superfamily domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGCCCCCTTTGCTGCTGCCGCTGCTGCCGCTGCTACTGCTGCTGCTGCT
Internal bar code:GCGTTAGAAGAAGTCTGTTTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:534
LEAP-Seq percent confirming:10.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:ACAGTCCCACTCACAGCATG
Suggested primer 2:CGCGTGTGTCATGTGTGTTT