| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.055537 |
| Chromosome: | chromosome 2 |
| Location: | 7169284 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g390467 | CGL66 | (1 of 3) PTHR12385:SF44 - PLASMA-MEMBRANE CHOLINE TRANSPORTER FAMILY PROTEIN; Conserved in the Green Lineage | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTGTTCCTGTATGTTCAGTGGACAAAAGATAAGTAAACAATGAGATGT |
| Internal bar code: | GGCCAGCCGAAAGCCGTGCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3453 |
| LEAP-Seq percent confirming: | 73.1183 |
| LEAP-Seq n confirming: | 68 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 93 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTATCGGGCAGCAAAATGG |
| Suggested primer 2: | TGTGTGTGTGTGTTTGTGCG |