| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.055561 |
| Chromosome: | plastome |
| Location: | 121904 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802313 | rbcL,ChreCp049,2717040 | (1 of 1) K01601 - ribulose-bisphosphate carboxylase large chain (rbcL); RuBisCO large subunit | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGTGACCTAGAGTACCACCACCGAACTGAAGACATGCGTCATCACCGA |
| Internal bar code: | CAAGTTACTGCAAGGGTGAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 146 |
| LEAP-Seq percent confirming: | 25.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAACCATTCATGCGTTGGC |
| Suggested primer 2: | TATACGTGCGACCCGATGTG |