| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.055566 |
| Chromosome: | chromosome 1 |
| Location: | 5059579 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g035250 | (1 of 1) PTHR21549:SF0 - COILED-COIL DOMAIN-CONTAINING PROTEIN 112 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATGGATTGGATTGCCCCCCACACACACACACACACGCTCCGTCCCACT |
| Internal bar code: | TAAACGTAGCCTGATGGCATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1953 |
| LEAP-Seq percent confirming: | 38.4615 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGGTAGTAGCATGGCTGAG |
| Suggested primer 2: | TTGTTGAGAAGGCAAGGGCA |