| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.055594 |
| Chromosome: | chromosome 1 |
| Location: | 3123522 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g019900 | FAP384 | Flagellar Associated Protein 384 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTTGCTGGGCGGCCCTGAGGAGGCTGCAGCATCCGGGAGCACAACATG |
| Internal bar code: | GATGGTGCGAGTATTGATAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1473 |
| LEAP-Seq percent confirming: | 55.0 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGGGCTTGGTGTTGACCT |
| Suggested primer 2: | CAAGTTATGGTCCCCCACCC |