| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.055597 |
| Chromosome: | chromosome 13 |
| Location: | 1130799 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g569500 | (1 of 3) PTHR10696 - GAMMA-BUTYROBETAINE HYDROXYLASE-RELATED; Similar to conserved hypothetical protein in bacteria | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCACCTGACGCCCGAGCACATCGCCGAGCTTGATGCAGCCGTGGACAAG |
| Internal bar code: | TAGATATCTATTAATACTGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3277 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGCACGAAGAGACAACCA |
| Suggested primer 2: | CGCATGTATGAACGGGCAAG |