Insertion junction: CLIP2.055601_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.055601
Chromosome:chromosome 8
Location:628109
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g360050 DUR1 Urea carboxylase/allophanate hydrolase; (1 of 1) 6.3.4.6 - Urea carboxylase / Urease (ATP-hydrolyzing) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGTGTGTGTGGGTTTGTTGGTTGCGCTTGCGTGTTTGTAAACTAGTTTG
Internal bar code:ATTGAATACAGAGTCACGTGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:460
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TGGGATGGTGCGCTTTATGT
Suggested primer 2:CAGTCCTGGATCGACGTCTG