| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.055603 |
| Chromosome: | chromosome 11 |
| Location: | 1296051 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467701 | intron | ||
| Cre11.g467702 | MRPS3,uS3m | (1 of 3) PF00189 - Ribosomal protein S3, C-terminal domain (Ribosomal_S3_C); Mitochondrial ribosomal protein S3 | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGATGCTCCACTGCCCGACTGCCCCGTGCCCGAGCCTGTCCTCCCCAGC |
| Internal bar code: | ACAAGGGCTTTAAGTAGACCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1165 |
| LEAP-Seq percent confirming: | 81.8182 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCCCTCGTTCACCGACAG |
| Suggested primer 2: | CATACAGGGCTACTCGCAGG |