| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.055608 |
| Chromosome: | chromosome 12 |
| Location: | 6462075 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g537550 | (1 of 7) 2.4.1.255 - Protein O-GlcNAc transferase / OGTase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGGTTCCCTGTTATTCTTGTTTTCTCACGGGATGCACTTTCTCCTCCT |
| Internal bar code: | TGGGTGGGAATGCGGGCCTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 987 |
| LEAP-Seq percent confirming: | 73.3333 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGTGTGTGTGTGTTCGG |
| Suggested primer 2: | GATGTTGTGTGGGAGGGGAG |