Insertion junction: CLIP2.055619_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.055619
Chromosome:chromosome 4
Location:2335436
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g220900 GT90-4,GT90F4 (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90); GT90 family protein 4 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTCATCGGAATTGGATGGAAACGAAGCCTTAAATTGTTGTGCCAACATG
Internal bar code:TTGCTTCTACGCTCGTACGCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:3003
LEAP-Seq percent confirming:46.1538
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CACCGTTTCTTGCCCTTGTG
Suggested primer 2:GACGAGTACAAGGTGGTGGG