Insertion junction: CLIP2.055619_3

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.055619
Chromosome:chromosome 4
Location:2335439
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g220900 GT90-4,GT90F4 (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90); GT90 family protein 4 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATAACTTGATAATCGCTATTTGCTCCCAGCCGGTGACACGTGCCGCATC
Internal bar code:TTGCTTCTACGCTCGTACGCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:2083
LEAP-Seq percent confirming:63.6364
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:GACGAGTACAAGGTGGTGGG
Suggested primer 2:CACCGTTTCTTGCCCTTGTG