Insertion junction: CLIP2.055626_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.055626
Chromosome:chromosome 16
Location:2284118
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g801907 CDS
lncRNA_TCONS_00176523 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTCATACGCACCAGACACGTGGGTCGGATTGGCGGAGGCGGCCAGTAGT
Internal bar code:TGTAAGTCTCAGGGAGGCTCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:417
LEAP-Seq percent confirming:94.1176
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:GGGCAAACAGATAGCATGCG
Suggested primer 2:ATATCGCGGTTCTTGGACCC