Insertion junction: CLIP2.055628_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.055628
Chromosome:chromosome 3
Location:5666757
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g186500 HAT2 Histone acetyltransferase 2; (1 of 1) K11303 - histone acetyltransferase 1 (HAT1, KAT1) CDS/intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCGTCAGATTGAGGCGGCCGGTCGCCACCGCCCCCGTGTCCGCCGCGC
Internal bar code:TCGTCCCATGACGGCCATGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1264
LEAP-Seq percent confirming:18.1818
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:GCCTTCATCAACGCGATCAC
Suggested primer 2:CCACATCCAGACTGCCCATT