| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.055659 |
| Chromosome: | chromosome 6 |
| Location: | 7361932 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g299950 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAACCTAGTCTGGGGCACTGTCCCGCTCACCGGTTCTTCTCCGGGTCAC |
| Internal bar code: | CCAACTCGGTGTGGTAGTACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3808 |
| LEAP-Seq percent confirming: | 47.0588 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 85 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACATAGAGGCGTGAGTCGG |
| Suggested primer 2: | CCTCGTCCAAGTCAACGTCA |