Insertion junction: CLIP2.055668_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.055668
Chromosome:chromosome 2
Location:7410640
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g392134 (1 of 3) 1.1.1.2 - Alcohol dehydrogenase (NADP(+)) / Aldehyde reductase (NADPH) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTCCCACCACCGTCCACACCGACCCACACCCAACCCACCGTTGACGATG
Internal bar code:TTTTAGGTTATTATTTATTGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1153
LEAP-Seq percent confirming:62.7119
LEAP-Seq n confirming:37
LEAP-Seq n nonconfirming:22
LEAP-Seq n unique pos:59

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTGATTATGCCACGCACG
Suggested primer 2:TGCTTCCTTCGTCATGTCCC