| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.055668 |
| Chromosome: | chromosome 2 |
| Location: | 7410642 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g392134 | (1 of 3) 1.1.1.2 - Alcohol dehydrogenase (NADP(+)) / Aldehyde reductase (NADPH) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTTGGAATCGTCGCAGGGCAAAGCGGCGCGTAGGCGTGTGTGGTTGAT |
| Internal bar code: | ATGTGTTAGCGGAGTAAAAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 137 |
| LEAP-Seq percent confirming: | 25.8065 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTTCCTTCGTCATGTCCC |
| Suggested primer 2: | CTGTGATTATGCCACGCACG |