Insertion junction: CLIP2.055672_3

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.055672
Chromosome:chromosome 12
Location:700885
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g492850 POC19 Proteome of centriole protein 19; (1 of 1) PF10595 - Uncharacterised protein family UPF0564 (UPF0564) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGCAAATGTATGGACGAAACAATCCAGTGCTTCGGCACTTTGGCGCTGA
Internal bar code:CAGGAAGTCAGTATCACCGAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:3677
LEAP-Seq percent confirming:93.1034
LEAP-Seq n confirming:27
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:GTCATAGGATCCAGGTGGCG
Suggested primer 2:AGGAGAGGACAATCGGACGA