| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.055674 |
| Chromosome: | chromosome 10 |
| Location: | 3451422 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g444400 | (1 of 2) K16458 - centrosomal protein CEP104 (CEP104) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGTGCCGTCGGACTGCGCGCCCACATAGTCGATCTTCTTGGGTGCTCG |
| Internal bar code: | GCAAACGAAATAGAAGGAGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3965 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGGCATTCGCTTAGAACG |
| Suggested primer 2: | GAATCATGGTTGCCTCGTGC |