| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.055677 |
| Chromosome: | chromosome 5 |
| Location: | 2198775 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g237600 | (1 of 1) PF09243 - Mitochondrial small ribosomal subunit Rsm22 (Rsm22) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGGAATGTGTTCAGGGCTGGGCTGCAAGGGCGCACGCACGCCGCACGG |
| Internal bar code: | TGGCATTGCATAGTCCCGACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 765 |
| LEAP-Seq percent confirming: | 16.6667 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTACCTGTTTGCGGACCAG |
| Suggested primer 2: | CAAGGGAAGAGTGGGCTGAG |