| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.055682 |
| Chromosome: | chromosome 6 |
| Location: | 8199288 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g306150 | (1 of 1) IPR000104//IPR004919 - Antifreeze protein, type I // Domain of unknown function DUF262 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCGGGCGCGCTCTGCGGCACCACCTTCTCCACGTGCAGGCCGCGGATG |
| Internal bar code: | AGAGTAAATATGAAGGTGCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 147 |
| LEAP-Seq percent confirming: | 18.1818 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGTCGGAGGTTCGGATTT |
| Suggested primer 2: | GCTGCTGTTAAGATCGCGTG |