| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.055717 |
| Chromosome: | chromosome 8 |
| Location: | 1265656 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g363750 | (1 of 2) K06268 - serine/threonine-protein phosphatase 2B regulatory subunit (PPP3R, CNB) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCAAATTAGCTCTGGGCGCGCAGAGCTCTGGCGCCTTGGGTTTAGAGC |
| Internal bar code: | GGAGATGGGTTAGAATCAAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 364 |
| LEAP-Seq percent confirming: | 6.66667 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCACATAACACCATCCCCA |
| Suggested primer 2: | CTCGCCAGCAAAGACCCTAA |