Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | - |
Strain: | CLIP2.055752 |
Chromosome: | chromosome 12 |
Location: | 2771773 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g504050 | LAG2 | Predicted protein related to longevity assurance protein; (1 of 5) 2.3.1.24 - Sphingosine N-acyltransferase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACCCGGAACGCCGCAGATACAAAATTAACGCGTGCTTGGCACACCGGA |
Internal bar code: | GCCTCTTGTAAAGCGTGCGCGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1980 |
LEAP-Seq percent confirming: | 46.8085 |
LEAP-Seq n confirming: | 44 |
LEAP-Seq n nonconfirming: | 50 |
LEAP-Seq n unique pos: | 94 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACAATCCTGGCTCAGCTCAC |
Suggested primer 2: | CCCATCCTTCTCAGCGCTAG |