Insertion junction: CLIP2.055755_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.055755
Chromosome:chromosome 13
Location:4677352
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g604050 (1 of 1) IPR002048//IPR006626//IPR011050 - EF-hand domain // Parallel beta-helix repeat // Pectin lyase fold/virulence factor CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACCGCGCTGGTGGGCTGTGACACGCGCGGCCAGGTGCGTGCACGTGCGC
Internal bar code:CACTGTGGTTTGACCCATCATG

Confirmation - LEAP-Seq

LEAP-Seq distance:1899
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:26
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:ATACACACAACAGGGCAGCA
Suggested primer 2:CACGTTTGCTGGTGTCCTTG