Insertion junction: CLIP2.055775_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.055775
Chromosome:chromosome 2
Location:4446919
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g106150 CYN18-1,CYN18A,CYN12 Cyclophilin 18-1; (1 of 1) K12734 - peptidyl-prolyl cis-trans isomerase-like 3 [EC:5.2.1.8] (PPIL3) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCCCGCCCCCCCGCCGCCAATAGCGCTACACCGCTTTACGCCATATCT
Internal bar code:ATATGGAGATGCCGTTTTCTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:2276
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:36

Suggested primers for confirmation by PCR

Suggested primer 1:CCACTCTCTCCGCACGATAC
Suggested primer 2:CAGTATTTTTCGGGCTCGCG