| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.055777 |
| Chromosome: | chromosome 12 |
| Location: | 3990313 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g515750 | NTP5,PHT4E,PHT4-5 | (1 of 3) PTHR11662//PTHR11662:SF223 - SODIUM-DEPENDENT PHOSPHATE TRANSPORTERS // SODIUM-DEPENDENT PHOSPHATE TRANSPORT PROTEIN 1, CHLOROPLASTIC; Na+-dependent inorganic phosphate cotransporter | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGTGAGCGGCTCTTGCCGCCTGCAGAGTGCATGGTCCTTTGGTGGAGAT |
| Internal bar code: | TTACGCAGGGAAGAGGGGGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3283 |
| LEAP-Seq percent confirming: | 98.7952 |
| LEAP-Seq n confirming: | 82 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 83 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAGGGTGTTGCGGATGGAC |
| Suggested primer 2: | TACATCAGGCTCAGGCTCCT |