Insertion junction: CLIP2.055778_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.055778
Chromosome:chromosome 7
Location:4194542
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g342050 NPSN1,VTI3 (1 of 1) K08494 - novel plant SNARE (NSPN); Endosomal Qb-SNARE, Npsn-family (plant/protist-specific) (Qb.III.d) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGGAAAAACCCTGCACCGTCGACCGACCATCATTGTTTCTACGAAAGGT
Internal bar code:GGTACGCTACGGTTCGCTTATT

Confirmation - LEAP-Seq

LEAP-Seq distance:1845
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:24
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:ACGACCAGACCAAGAAGCTG
Suggested primer 2:CACGTGAACACAACACACCC