| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.055784 |
| Chromosome: | chromosome 1 |
| Location: | 6115128 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g043400 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATTCCAAGCTGGTTCTGGATACAAACAGCAGACAGACTGCGTGGATTG |
| Internal bar code: | CTGCACGTGGACTGCAGGCTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 410 |
| LEAP-Seq percent confirming: | 73.3333 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGGTATGCGCCTCAAAAG |
| Suggested primer 2: | CAAAGAGGAGGAGGATGCGG |