| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.055785 |
| Chromosome: | chromosome 14 |
| Location: | 3758455 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g631850 | (1 of 1) 3.1.3.74 - Pyridoxal phosphatase / Vitamin B6-phosphate phosphatase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAACGTTGGCCGGTGTGCCGACCAGCCAGGATCGCGGCGCCGCCCCCA |
| Internal bar code: | TCGGTGGGATAACTGGGGGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3293 |
| LEAP-Seq percent confirming: | 97.561 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGCTCACAGCAACTCCTC |
| Suggested primer 2: | ACAAGTGTCCTAACAGCCCG |