| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.055789 |
| Chromosome: | chromosome 6 |
| Location: | 5135892 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g283100 | FAP85 | Flagellar Associated Protein 85; (1 of 140) IPR002048 - EF-hand domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGGGCCAGCCGCTGGTGGGGCTTGCTAAGCCCGTGCTGGAGGTAGGCA |
| Internal bar code: | TGTGTTGTGACACAGCATTGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1958 |
| LEAP-Seq percent confirming: | 76.7857 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAGTGGCAAGGCAAGTTG |
| Suggested primer 2: | AGGATGTCCTACAGGCGAGT |