| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.055804 |
| Chromosome: | chromosome 6 |
| Location: | 2926166 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g273200 | (1 of 1) K03762 - MFS transporter, MHS family, proline/betaine transporter (proP) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTACTTACAACCCTCGGGACCGCCATGTGCGCCTGGTGTGCGCCTGCAGG |
| Internal bar code: | CTCTACGTAGGGGTAGCGTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2876 |
| LEAP-Seq percent confirming: | 83.5821 |
| LEAP-Seq n confirming: | 56 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 67 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCACAAACTTACACGCCC |
| Suggested primer 2: | CACTCGGAAGCAGGTCAAGT |