Insertion junction: CLIP2.055844_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.055844
Chromosome:chromosome 14
Location:2413677
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g623439 (1 of 1) 3.4.19.3 - Pyroglutamyl-peptidase I / Pyrrolidone-carboxylate peptidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTGGCACTCGCTCTTCATTCATGTGCCGCCATTCAGTGTGTATGACGAG
Internal bar code:CGCACCTTCTAGCTTTCGTGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1401
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:TACGTGGTGGCGTTTCATCA
Suggested primer 2:TGTGGTGCTTGCGTTTGATG