| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.055857 |
| Chromosome: | chromosome 7 |
| Location: | 4098832 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g342900 | (1 of 2) 2.7.1.100 - S-methyl-5-thioribose kinase / MTR kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCACCACGACCTCCAGCAGCGCCGCCGCCAGCTGCAGCCCGCTGCTATC |
| Internal bar code: | CATGCGGTTTAATGGTTTTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 967 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATTTCGTGCGATGCTGAT |
| Suggested primer 2: | GGGGGAGGGAGAATGGTTTG |