Insertion junction: CLIP2.055863_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.055863
Chromosome:chromosome 6
Location:2770484
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g271400 PGP3 (1 of 3) 3.1.3.18 - Phosphoglycolate phosphatase; Phosphoglycolate phosphatase/4-nitrophenylphosphatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGACACACACTCCCATCTCCCATCAGCTGGGCTCCAGGCTCTGTGCGGC
Internal bar code:ATCTTCGAATCTGTGGCCCTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1023
LEAP-Seq percent confirming:24.4898
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:37
LEAP-Seq n unique pos:49

Suggested primers for confirmation by PCR

Suggested primer 1:GTTCACACACTCACACACGC
Suggested primer 2:GTCATGCCCAGAAATCCCCA