| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.055866 |
| Chromosome: | chromosome 2 |
| Location: | 1572577 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g084800 | SPO11A | (1 of 1) K10878 - meiotic recombination protein SPO11 (SPO11); meoisis-related DNA topoisomerase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGGTTCTCTTTCTGGCGTCGCTCATCCAGGCTTGCCGTCCGGCCCCAG |
| Internal bar code: | CCTATGGGCCCATGTGGGACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1776 |
| LEAP-Seq percent confirming: | 73.3333 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGATGATGCAGGGGTAGGT |
| Suggested primer 2: | CAAACTGTTTGAGGTGCGGG |