| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.055868 |
| Chromosome: | chromosome 7 |
| Location: | 930897 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g319002 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGGTGCTGCCGCTGACGGTGCTGGCAGTGCCGGTGCTGTTGGTACCAG |
| Internal bar code: | GCTTCCTGGTGTGGGCACTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 553 |
| LEAP-Seq percent confirming: | 4.34783 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTTATGTGTGACGGCATC |
| Suggested primer 2: | AGACGTACGTGGTGCAACAT |