| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.055879 |
| Chromosome: | chromosome 10 |
| Location: | 1150254 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g425600 | PGM15 | Haloacid dehalogenase-like hydrolase (HAD) superfamily protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCCGCTGGGTAGGGCGCTGCGCGGCTGCTGCATTCCGCCGCCGGCTC |
| Internal bar code: | GGTTCGCGCCGGGAATCGCTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1297 |
| LEAP-Seq percent confirming: | 81.0811 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGGAGGAGGAGGGAAAC |
| Suggested primer 2: | GTTGTACTGCCCGCCATACT |