| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.055882 |
| Chromosome: | chromosome 16 |
| Location: | 6743988 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g672750 | (1 of 2) K14944 - RNA-binding protein Nova (NOVA) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAACTCCCGACAGCGGCACCCAACCCCCGCCACGTACCGCGTCCTCGGGC |
| Internal bar code: | GTTCGGTAAGTAATACGTGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 877 |
| LEAP-Seq percent confirming: | 47.8261 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATTCCATCGCTGGTTCGGT |
| Suggested primer 2: | CGAGCGCAGATGGGAACTAT |